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AAS Method Development & System Proficiency Training

Following our proficient on-site engagement, your staff won't just be certified; They will be advanced operators, fully equipped with the knowledge and confidence that guarantee the continuity of your lab's high-performance operation.

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Module 1: AAS Fundamentals and Instrumentation

Principles of Atomic Absorption
- Understanding the absorption of light by ground-state, unexcited atoms
- The Beer-Lambert Law (A=ϵbc) as the quantitative basis for measurement.
AAS Light Sources
- Detailed function of the Hollow Cathode Lamp (HCL): operation and need for a separate lamp per element
- Use and advantages of Electrodeless Discharge Lamps (EDLs)
- Proper selection of lamp current and slit width.
Optics and Detectors
- Single vs. Double-Beam optics and why double-beam is preferred for stability
- Function of the Monochromator and Photomultiplier Tube (PMT)
- Necessity and methods for Background Correction (D2 Arc and Zeeman Effect)

Module 2: Flame AA (FAAS) - Operation and Method Development

FAAS Atomization System
- The Nebulizer/Spray Chamber assembly: function, maintenance, and impact on efficiency
- Burner Head types (10 cm for Air/Acetylene and 5 cm for Nitrous Oxide/Acetylene) and their applications
- Safe handling and operation of fuel and oxidant gases.
FAAS Interferences and Correction
- Spectral Interferences: Identifying background absorbance (D2 correction)
- Chemical Interferences: Strategies for suppression (e.g., using releasing agents like Lanthanum or Strontium)
- Ionization Interferences: Using Ionization Suppressants (e.g., Potassium) to shift the ionization equilibrium.
Method Optimization for FAAS
- Optimizing Burner Height and Gas Flow Ratios for maximum signal
- Selecting the correct analytical wavelength and slit setting
- Techniques for measuring high-solids or organic solvent samples.

Module 3: Graphite Furnace AA (GFAAS) - Advanced Techniques

GFAAS Furnace and Autosampler
- Components of the Graphite Furnace
- Function and importance of the Autosampler for precise injection and dispensing
- Understanding the role of Graphite Platforms and coating for signal enhancement
Temperature Program Optimization
- Detailed study of the time/temperature program: drying, pyrolysis (ashing), atomization, and cleaning steps
- Optimizing the pyrolysis temperature to remove matrix without losing the analyte
- Optimizing the atomization temperature for maximum absorbance and sensitivity.
Matrix Modification and Interference
- Principles and application of Matrix Modifiers (e.g., Pd and Mg) to stabilize the analyte
- Utilizing the Zeeman Background Correction method for superior background removal in complex matrices

Module 4: System Maintenance, Troubleshooting, and Compliance

Routine Maintenance
- Flame AA PM: Nebulizer cleaning, spray chamber disassembly, and burner slot maintenance
- GFAAS PM: Graphite Tube conditioning, replacement, and electrode cleaning
- Checking and replacing AAS-specific consumables (HCLs, graphite components)
Troubleshooting
- Diagnosing Low Sensitivity/High Noise in Flame AA (e.g., clogged nebulizer)
- Correcting Poor Precision in GFAAS (e.g., tube life, inconsistent dispensing)
Validation and Quality Control
- Method validation in AAS: determining Characteristic Concentration and Detection Limits (DLs)
- Proper use of Quality Control (QC) standards and Spike Recoveries

Training syllabus

This course delivers expertise in AAS System Proficiency and robust method development for both Flame (FAAS) and Graphite Furnace (GFAAS) techniques. You'll gain practical skills to ensure guaranteed compliance and data integrity, alongside advanced maintenance and troubleshooting for sustained, high-quality performance.

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