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Single Cell ICP-MS

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Single Cell ICP-MS Method Development & System Proficiency Training

Following our proficient on-site engagement, your staff won't just be certified; They will be advanced operators, fully equipped with the knowledge and confidence that guarantee the continuity of your lab's high-performance operation.​

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Module 1: SC-ICP-MS Sample Handling & Instrument Interface

  • Cell Preparation and Suspension

    • Maintain cell integrity (culturing, washing, pH) to prevent lysis

    • Select appropriate dispersion Media (buffers, TDS) for stability

  • Advanced Sample Introduction

    • Optimize low-flow nebulizers for maximum cell transport efficiency (ηcell​)

    • Utilize Specialized Accessories (single-cell spray chambers).

  • SC-ICP-MS Instrumental Tuning

    • Optimize fast time-resolved analysis (TRA) by fine-tuning dwell times (≤100μs)

    • Correct for background noise from the biological matrix/media

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Module 2: Quantitative Method Development

  • Elemental Mass Calibration per Cell

    • Calibrate signal (counts) to analyte mass (attogram/fg) using dissolved standards

    • Use Internal Standardization to correct for biological matrix effects

    • Accurately measure cell transport efficiency (ηcell​).

  • Multi-Element Quantification

    • Optimize parameters for measurement of multiple elements

    • Use ICP-MS/MS (Triple Quad) to remove complex polyatomic interferences in biological matrices

  • Cell Marker Quantification

    • Use endogenous elements (P,S) for cell mass normalization

    • Calibrate a ubiquitous marker for absolute marker quantification (average cell mass).

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Module 3: Advanced Data Processing and Interpretation

  • Data Filtering and Noise Reduction

    • Implement Advanced Thresholding (dual-thresholding) to separate cell events from noise

    • Manage signals from non-ideal or lysed cells

    • Utilize specialized software for efficient batch processing.

  • Statistical Analysis of Cell Populations

    • Analyze Population Heterogeneity using histograms to show cell-to-cell variability

    • Report key metrics: median mass per cell and Geometric Standard Deviation (GSD)

  • Lysis Correction and Validation​

    • Apply mathematical correction models to account for dissolved background from lysed cell contents

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Module 4: SC-ICP-MS Applications and Future Trends

  • Pharmacokinetics and Drug Uptake

    • Quantify cellular dose of metal-based drugs (e.g., Cisplatin) in cells

    • Measure and compare drug uptake across cell lines and time points.

  • Immuno-labeling and Cell Tagging

    • Compare principles of Mass Cytometry (CyTOF) vs. SC-ICP-MS

    • Apply metal-labeled antibodies to quantify multiple cell surface markers

  • Emerging Techniques and Regulatory Outlook

    • Introduce Microfluidics coupled to SC-ICP-MS

    • Review the path toward standardization and validation in clinical/ecotoxicology labs.

Training syllabus

This course delivers expertise in SC-ICP-MS system optimization and advanced quantitative method development, focusing on cell handling, elemental mass calibration, and multi-element analysis. You will gain practical skills in advanced data processing (including thresholding and lysis correction) and master the interpretation of cellular population heterogeneity for biological applications.

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